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KMID : 0379520060220010015
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한국독성학회지
2006 Volume.22 No. 1 p.15 ~ p.22
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Steroid Hormone Receptor/Reporter Gene Transcription Assay for Food Additives and Contaminants
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Jeong Sang-Hee
Cho Joon-Hyoung
Park Jong-Myung
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Abstract
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Many of endocrine disrupting chemicals induce effects via interaction with hormone receptors and responsive elements in target cells. We investigated endocrine disrupting effects of some food additives and contaminants including BHA, BHT, ethoxyquin, propionic acid, sorbic acid, benzoic acid, CPM, aflatoxin B1, cadmium chloride, genistein, TCDD and PCBs in yeast transformants expressing human steroid hormone receptors along with steroid responsive elements. The response limit of genetically recombinant yeast to 17{beta}-estradiol, testosterone and progesterone was 1{times}10^{-16},;1{times}10^{-12};and;1{times}10^{-13}M, respectively. BHT induced weak transcriptional activity in estrogen sensitive yeast, while BHA and sorbic acid interacted weakly with androgen receptor/responsive element. CPM induced transcriptional activities in all types of yeasts sensitive to steroid hormones. Zearalenone and genistein induced high transcriptional activation in estrogen sensitive yeast with relative potencies almost 10^8 folds lower than 17{beta}-estradiol. TCDD induced transcriptional activation weakly in estrogen- and progesterone- sensitive yeasts. This study elucidated that recombinant yeast is a sensitive and high-throughput system and can be used for the direct assessment on chemical interactions with steroid receptors and responsive elements. Also, the present study raises the requirement of evaluation on the endocrine disrupting effects of BHT, BHA, sorbic acid, CPM and TCDD for their transcription activity in yeast screening system though weak in intensity.
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KEYWORD
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Endocrine disruptor, Food additives, Food contaminants, Gene transcription assay, Steroid hormone
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